Catalytic activity and conformation of chemically modified subtilisin Carlsberg in organic media

1999 ◽  
Vol 66 (4) ◽  
pp. 265-270 ◽  
Author(s):  
Oh Hyeong Kwon ◽  
Yukio Imanishi ◽  
Yoshihiro Ito
Keyword(s):  
Catalytic Activity ◽  
Organic Media ◽  
Chemically Modified ◽  
Subtilisin Carlsberg

scholarly journals Ultra-Small Pd(0) Nanoparticles into a Designed Semisynthetic Lipase: An Efficient and Recyclable Heterogeneous Biohybrid Catalyst for the Heck Reaction under Mild Conditions

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2358 ◽  
Author(s):  
David Lopez-Tejedor ◽  
Blanca de las Rivas ◽  
Jose M. Palomo
Keyword(s):  
Catalytic Activity ◽  
Protein Structure ◽  
Heck Reaction ◽  
Catalytic Performance ◽  
Reducing Agent ◽  
Chemically Modified ◽  
Mild Conditions ◽  
In Situ Formation ◽  
Geobacillus Thermocatenulatus

A novel heterogeneous enzyme-palladium (Pd) (0) nanoparticles (PdNPs) bionanohybrid has been synthesized by an efficient, green, and straightforward methodology. A designed Geobacillus thermocatenulatus lipase (GTL) variant genetically and then chemically modified by the introduction of a tailor-made cysteine-containing complementary peptide- was used as the stabilizing and reducing agent for the in situ formation of ultra-small PdNPs nanoparticles embedded on the protein structure. This bionanohybrid was an excellent catalyst in the synthesis of trans-ethyl cinnamate by Heck reaction at 65 °C. It showed the best catalytic performance in dimethylformamide (DMF) containing 10–25% of water as a solvent but was also able to catalyze the reaction in pure DMF or with a higher amount of water as co-solvent. The recyclability and stability were excellent, maintaining more than 90% of catalytic activity after five cycles of use.

Ultrathin Anionic Tungstophosphite Molecular Wire with Tunable Hydrophilicity and Catalytic Activity for Selective Epoxidation in Organic Media

2017 ◽  
Vol 23 (69) ◽  
pp. 17497-17503 ◽  
Author(s):  
Zhenxin Zhang ◽  
Masahiro Sadakane ◽  
Michikazu Hara ◽  
Wataru Ueda
Keyword(s):  
Catalytic Activity ◽  
Organic Media ◽  
Molecular Wire

Novel microbial lipases: catalytic activity in reactions in organic media

2001 ◽  
Vol 28 (2-3) ◽  
pp. 145-154 ◽  
Author(s):  
F. Cardenas ◽  
M.S. de Castro ◽  
J.M. Sanchez-Montero ◽  
J.V. Sinisterra ◽  
M. Valmaseda ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Organic Media ◽  
Microbial Lipases
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